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In vitro antifungal activity of Lippia graveolens and Viscum album ethanolic extracts against Fusarium oxysporum f. sp. lycopersici
Vascular wilt caused by Fusarium oxysporum f. sp. lycopersici is a problem in tomato crops. Its management is commonly done with synthetic products. However, the excessive use of such products could induce resistance to the active ingredients and toxicological problems. Because of this, biorational alternatives such as plant extracts have been proposed for plant disease management. The objectives of this research were to obtain information on the phytochemical composition of plant extracts, as well as to determine the antifungal activity and the inhibitory concentration at 50 and 90 % (IC50 and IC90) of ethanolic plant extracts of Lippia graveolens and Viscum album using the microdilution plate method. A Probit analysis was performed to determine the IC50 and IC90 of each extract, and ANOVA by Tukey’s test (p<0.05) to determine statistical differences. Phytochemical compounds, such as hydroxycinnamic acids, stilbenes, flavonoids, catechins, polyphenols, and methoxycinnamic acids were identified in both extracts. Furthermore, both extracts showed antifungal activity higher than 80 % inhibition. However, the L. graveolens extract was statistically superior, with the lowest IC50 of 60.91 mg/L. In conclusion, both plant extracts contain phytochemical compounds of interest for the control of microorganism and represent a natural alternative for controlling F. oxysporum f. sp. lycopersici.
Keywords: Botanical fungicides, Plant extracts, Phytochemical compounds, Biological control of diseases, Biofungicides.